To develop simple and economical UV spectrophotometric method for the simultaneous estimation of Olopatadine HCl and Ketorolac tromethamine in the combined ophthalmic dosage form available in the market for the prophylaxis of conjunctivitis. Two drugs Olopatadine HCl and Ketorolac tromethamine are present in the ratio of 1:4. The normal overlay spectra of two drugs in distilled water revealed an isoabsorptive point at 263 nm. The other wavelength selected was λmax of Olopatidine HCl (249 nm). The method was validated as per the ICH guidelines. Beer’s law followed in the range of 8-45 µg/ml for both Olopatadine HCl and Ketorolac tromethamine. Recovery studies by standard addition method were in the range of 97% to 101%. The relative standard deviation for intraday and interday precision was found to be less than 2% indicating the method to be repeatable. A simple, accurate, sensitive and economical UV-Spectrophotometric method for the simultaneous estimation of Olopatadine HCl and Ketorolac tromethamine in combined dosage form has been developed which can be employed in the industry for the routine analysis.
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